Differentiating 3t3 - l1 preadipocytes undergo clonal expansion induced by acylation stimulating protein 前脂肪細(xì)胞分化為成熟脂肪細(xì)胞后的再增殖
Thus , it is evident that 3t3 - l1 preadipocytes could differentiate into adipocytes without dna synthesis and mitotic clonal expansion 這些結(jié)果說(shuō)明克隆擴(kuò)增和dna復(fù)制并不是3t3 l1前脂肪細(xì)胞分化的必需步驟。
By inducing 3t3 - l1 preadipocyte with individual hormone , it was identified that the mitotic clonal expansion was induced only by insulin , not by 1 - isobutyl - 3 - methylxanthine or dexamethasone 有報(bào)道用藥物抑制了克隆擴(kuò)增的同時(shí)抑制了細(xì)胞的分化。我們發(fā)現(xiàn)克隆擴(kuò)增是僅由胰島素誘導(dǎo)引起的。
However , significant fraction of 3t3 - l1 preadipocytes differentiated into adipocytes without the mitotic clonal expansion when the preadipocytes were induced with the combination of 1 - isobutyl - 3 - methylxanthine and dexamethasone 用地塞米松和ibmx對(duì)細(xì)胞進(jìn)行誘導(dǎo)不發(fā)生克隆擴(kuò)增,而細(xì)胞也能部分分化,分化形成的脂肪細(xì)胞未經(jīng)過(guò)dna復(fù)制。
Furthermore , when normally induced 3t3 - l1 preadipocytes were treated with pd98059 ( an inhibitor of mek - 1 ) , to block the activation of erk1 and 2 , the mitotic clonal expansion was blocked , while adipocyte differentiation was not affected 我們用有絲分裂原激活蛋白激酶激酶( mapkk / mek1 )的抑制劑pd98059與誘導(dǎo)分化激素及藥物一起對(duì)細(xì)胞誘導(dǎo)分化,克隆擴(kuò)增不發(fā)生,但細(xì)胞分化未受影響。